Methodological exploration of improving the survival rate of human fetal hepatocytes transplanted into nude mice

Abstract

Objectives To explore whether human fetal hepatocytes(HFH)transplanted into nude mice survived and study the methods for raising successful rate of transplantation.Methods The suspension of human fetal hepatocytes was transplanted into the abdominal cavity.liver.speen and mesenterium respectively.Three months later,histological observation was performed.Results The HFH could be found on the transplanting sites of spleen and mesenterium with only 10% successful rate of transplantation. Intraperitoneal injection of dexamethasone after transplantation could increase the Successful rate of the transplantation to 6/10.Under this circumstances.if HFH in cellular Sphere culture was used as transplantation material,successful rate of transplantation could reach 90%.Based on methods mentioned above,a lobe of liver in nude mice was resected,successful rate of transplantation could reach 100%,and HFH transplanted into the spleens of nude mice still survived 6 months after transplantation.Conclusions HFH transplanted in nude mice could survive for at least 6 months.If transplantationmaterials were made up into cellular sphere cultures,combining with using dexamethasone,the survival rate of transplantation might significantly be increased. Key words: Cell transplantation; Mice,nude; Embryo; Liver; Graft survival