Abstract
Strong evidence exists that the distribution of phospholipids in the lung is a function of the degree of adult respiratory distress syndrome. The capabilities of positive-ion fast-atom bombardment (FAB) mass spectrometry for the determination of the relative distribution of intact diacylphosphatidylcholine species in lung lavage fluid were investigated. Two different FAB matrices and two different isolation/purification procedures--extraction/thin layer chromatography--high performance liquid chromatography (TLC-HPLC)--have been evaluated. In addition the relative fatty acid composition of the diacylphosphatidylcholines was determined by negative-ion FAB mass spectrometry using the [RCOO]- fragment ion. These results were compared with those obtained by gas chromatographic determination of the fatty acid methylesters.
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