Method Verification and Measurement of Uncertainty Estimation for the Proximate Analysis in Animal Feed-Single Laboratory Verification Protocol (Nordtest Approach)

Abstract

The purpose of this study was to verify the proximate analysis (DM percent, Ash percent, CP percent, EE percent, and CF percent) using AOAC approved methods at the Feed Quality Control Laboratory, DLS, Savar, Dhaka, and to reduce measurement uncertainty in each test in the laboratory. To verify the test procedures, a laboratory-made control sample (full-fat soybean), a certified control sample, and two Proficiency test feed samples (List A) were employed, together with various calibrate equipment (Table 1,2). This strategy was put to the test in terms of precision, accuracy, and consistency. Limits of Detection (LOD), Limits of Quantification (LOQ), Robustness, and Ruggedness, on the other hand, are irrelevant in these verification and uncertainty assessment techniques. Tests were carried out over a period of several months to verify this process and to assess the uncertainty more precisely. The Recovery percent was determined for the accuracy test, the Nordtest technique (Single Laboratory Verification Technique), and FAO. 2011 guidelines were used to assess the precision of the methods’ long-term repeatability. The recovery% of the determination of DM%, Ash%, CF%, EE%, and CP% of the Laboratory manufactured Reference sample, FAPAS Proficiency test sample-1, and Sample 2 (Tables 3-7) were within 98-102%, while the precision was determined by long-term repeatability (SRw) was 0.87%, 0.13%, 0.29%, 0.25%, and 0.35%, respectively, which met the criterion (Table 2) of FAO guideline. The measurement uncertainty was computed using the Nordtest method (Single Laboratory Verification Technique) and FAO. 2011 criteria, based on the longterm repeatability of the proximal components in the acknowledged laboratory. According to the guideline, the Expanded Measurement Uncertainty for DM percent, Ash percent, CF percent, EE percent, and CP percent was 0.99, 0.89, 1.02, and 1.82 percent, respectively, which was quite satisfactory. So, in the respective laboratory-Feed Quality Control Laboratory, Department of Livestock Services, Savar, Dhaka, Bangladesh-this verification for proximate components analysis of the animal feed is validated.