Abstract
A quick qualitative technique was developed for detection of the soluble antigen (Ag) and antibody (Ab) complex formed as the result of union between Ag and Ab from solution. A drop 05 each sample is placed on a microscope slide coated with silver; the nonmetalized side of the slide is resting on the microscope stage. The other slide is positioned very gently in place to form a wedge, and an interferometric fringe is formed by using a low-power helium-neon gas laser (6328 A) as monochromatic light source. If Ag and Ab are specific, the precipate complex and its growth can be observed within five minutes. The short time requirement is an advantage over other immunodiffusion methods.
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