Abstract
In recent years extensive ultrastructural studies of the pulmonary system have been published. Many of these studies have been initiated to clarify the effects of air pollutants and inhaled gases on tracheal mucosa, intrapulmonary air passages and alveoli. Although light microscopic evaluation of the nasal mucosa has been included in some recent morphologic studies of the effects of inhaled toxicants, the ultrastructural effects on nasal mucosa have rarely been published. This lack of information is primarily due to the difficulty of preparing the epithelial mucosa for examination since it is completely encased in bone. We have developed a detailed systematic method to prepare any site in the rat nasal mucosa for ultrastructural evaluation by both transmission and scanning electron microscopy.Fixation is achieved by an intravascular perfusion technique using meto- fane (Pitman Moore, Inc.) anesthetized 200 gram Fischer-344 rats. The perfusing catheter is inserted through the left ventricle and into the ascending aorta.
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