Abstract
A rapid and sensitive analytical method for the determination of isoxaflutole and diketonitrile (DKN) residues in maize was established based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) and high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). The samples were extracted with acetonitrile and purified with octadecylsilane (C<sub>18</sub>). The mass spectrometer was performed in multi-reaction monitoring mode, and electrospray ionization in negative mode was selected. The isoxaflutole and DKN residues were quantified by the external standard method using the matched standard solution to compensate for matrix effect. The results indicated that the calibration curves of isoxaflutole and DKN were linear in the range of 0.005-0.5mg/L with correlation coefficients of more than 0.9922. When the addition level ranged from 0.01 to 0.1mg/kg the average recoveries of isoxaflutole and DKN in maize, green maize and straw were 95%-108%, and the relative standard deviation (RSD) ranged from 2% to 11%. The limit of quantitation (LOQ) were 0.01 mg/kg in different matrices. This method has many characteristics, such as simple, rapid and accurate, and can be adapted for the confirmation of isoxaflutole and DKN residues in maize samples.
Highlights
Isoxaflutole (Figure 1) is a selective pre-emergent herbicide, which is mainly used in the field of corn and sugarcaneto control various annual broadleaf weeds and some gramineous weeds
The mode of action of isoxaflutole is the inhibition of the enzyme 4-hydroxyphenylpyruvate dioxygenase (HPPD), thereby inhibiting pigment formation, and causing bleaching of the developing tissues of the weeds [1]
The registration of isoxaflutole on maize is being carried out in our country, so it is urgent to establish the method of residue analysis on maize and maize straw
Summary
Isoxaflutole (Figure 1) is a selective pre-emergent herbicide, which is mainly used in the field of corn and sugarcaneto control various annual broadleaf weeds and some gramineous weeds. There is few reports on the methods for analysis of isoxaflutole and its metabolites residues, mainly involving water, soil and forage plant [6,7,8]. Yang Changzhi et al [9] established a HPLC-MS/MS method for simultaneous determination of isoxaflutole and DKN in maize by APCI source combined with QuEChERS, but it did not involve maize straw. China has not established the maximum residue limit of isoxaflutole inmaize [10], while CAC, the United States, Japan and European Union all set the maximum residue limits (0.02mg/kg) for maize. The residue analysis method of isoxaflutole and DKN, in maize and maizestraw was establishedby HPLC-MS/MS. Mao Jia et al.: Method for Residues Analysis of Isoxaflutole and Its Metabolites in Maize
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