Abstract

AbstractA method has been developed for the quantitative determination of isothiocyanates and oxazolidinethiones in enzymic digests of seed meals of rape (Brassica napus) and turnip rape (B. campestris). Good precision has been obtained; relative standard deviation was ±2% when the method was applied with macro‐ (1 g) as well as on micro‐ amounts (10 mg) of seed meal.In order to obtain optimum yields from the same digests, the seed and meal were treated with hot air and hot buffer solution, respectively. The time/temperature/moisture relationship of seed treatment necessary to inactivate the myrosinase in rape seed is presented.The results obtained with this method demonstrate that the content of progoitrin in rape seed is considerably higher than that obtained by application of an earlier widely used method, e.g. 4.8% in defatted seed meal of Matador winter rape, compared with an earlier figure of 3.1%.

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