Abstract
Laccaria laccata and Suillus bovinus were grown on cellophane membranes on Petri dishes of thick half-strength modified Melin-Norkrans' agar for up to 70 d. Mycelium was then removed from the outer, middle or inner areas of the colonies and analysed for chitin. The technique avoided problems associated with liquid culture and provided mycelium free from agar which facilitated estimations of conversion factors (chitin content per unit dry weight (d.w.)) on different age ranges of mycelium. Evidence of the potential of such a membrane based growth system, for providing conversion factors, for use in estimating the chitin content of ectomycorrhizal species and other fungi when grown on solid substrates, is provided.
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