Abstract
The study presents the method for simultaneous determination of selenium and arsenic in human urine by atomic fluorescence spectrometry (AFS). According to the procedure developed, a sample is first digested in the microwave system, then chemically treated in the flow through a hydride generation system, and finally exposed to measurements in a double‐channel atomic fluorescence spectrometer. It has been revealed that selenium and arsenic can be accurately determined with detection limit of 0.13 and 0.16 µg/L and repeatability (RSD) of 1.0 and 1.2%, respectively. The urine samples taken from a control group and from persons subjected to a special diet were analyzed. The obtained results proved that the method developed was capable of controlling reliably even slight changes of both elements in a wide range of their concentrations, and, as such, that it can be recommended to be used for clinical and toxicological purposes.
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