Abstract
A temperature perturbation in biological systems has been performed in a simple apparatus. A 5° jump in 0.5 to 0.7 ml of cell suspension was obtained with a 200 joule xenon flash within 400 msec. The jump was “chemically pure” and little or no damage of cells was detected microscopically. The response of cellular NADH to the temperature jump was an initial fast decrease in its fluorescence efficiency, followed by a delayed NADH oxidation with a half-time of several seconds. No more rapid biological relaxation was observed under the conditions studied. The advantages of using a flash heating and of a current pulse heating are discussed.
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