Abstract
In this present work two colorimetric methods were developed based on donor-acceptor complexes of alverine citrate (ALV) and tapentadol (TAP) with cobalt thiocyanate. These methods were developed on Perkin Elmer LAMBDA 25 UV–VIS spectrophotometer with 1cm quartz cells. The colored species formed are the coordination complexes of the drugs (electron donor) and the central metal atom of cobalt thiocyanate (electron acceptor) which is extractable into nitrobenzene from aqueous solution. The reaction conditions were optimized and validated to achieve maximum colour intensity. The colored complexes show maximum absorbance measured at 625 nm for both ALV and TAP. The absorbances were found to increase linearly with an increase in concentration which was corroborated by the calculated regression coefficients (0.9998-0.9999). Linearity was obeyed in the range of 100-600 µg/ml for both ALV and TAP, respectively. The molar absorptivity, sandell’s sensitivity, LOD, LOQ and other validation parameters have been evaluated extensively as per ICH guidelines and all the parameters were found within the acceptance criteria for both methods. The proposed methods were proven to be more accurate, simple, precise and rapid by statistical validation of recovery studies and could be suitable for regular analysis.
Highlights
Alverine citrate (British Pharmacopoeia, 2008) chemically is N-Ethyl-N-(3-phenyl propyl)-benzene propanamine (Fig. 1) called as spasmaverine used as an antispasmodic by directly acting on the muscle in the gut to relax
In this present work two colorimetric methods were developed based on donor-acceptor complexes of alverine citrate (ALV) and tapentadol (TAP) with cobalt thiocyanate
Literature is enriched with several techniques like HPLC and spectrophotometry for the determination of TAP (Asha et al, 2012; Babu and Madhu, 2012; Deepti and Pawan, 2013; Indira et al, 2013; Krishanmurthy et al, 2014; Neol et al, 2009; Omkar and Priti, 2012; Rizwana et al, 2014; Suresh et at., 2013) and ALV( Vijayalakshmi et al, 2014; Ghosh et al, 2010; Rahul et al, 2011; Kumar et al, 2013)
Summary
Alverine citrate (British Pharmacopoeia, 2008) chemically is N-Ethyl-N-(3-phenyl propyl)-benzene propanamine (Fig. 1) called as spasmaverine used as an antispasmodic by directly acting on the muscle in the gut to relax. In this present work two colorimetric methods were developed based on donor-acceptor complexes of alverine citrate (ALV) and tapentadol (TAP) with cobalt thiocyanate. The colored species formed are the coordination complexes of the drugs (electron donor) and the central metal atom of cobalt thiocyanate (electron acceptor) which is extractable into nitrobenzene from aqueous solution.
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