Abstract
Development and validation of simple, rapid, accurate, precise and sensitive UV-Spectrophotometric method for the estimation of Ephedrine hydrochloride in bulk drug and Injection dosage form was performed in the current research. Quantitative determination of Ephedrine hydrochloride was done using distilled water as a solvent. λmax of Ephedrine hydrochloride in distilled water was measured at 270 nm. Linearity range for Ephedrine hydrochloride was 2-10 μg/mL and coefficient of correlation for Ephedrine hydrochloride was 0.999. Accuracy was performed and the percentage recovery of Ephedrine hydrochloride was found to be in the range of 98.6-99.17. The % relative standard deviation (RSD) for precision was less than 2%, LOD & LOQ was 0.079 μg/mL and 0.24 μg/mL respectively. The results suggest that method can be employed for routine analysis of Ephedrine hydrochloride in bulk drug and in pharmaceutical Dosage form.
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