Abstract
The aim of the present work is to develop a new, simple, sensitive, accurate and analytical method of developing and validating the stability indicating estimation of felodipine in pharmaceutical tablet dosage form using RP-HPLC. The API was supplied by Aurobindo Pharma Ltd, Hyderabad. The chromatographic separation was done with Inertsil ODS _2 C_18 (100 × 4.6mm, 3μ) using HPLC Shimadzu (2010 CHT) instrument with LC solutions software. The suitable wavelength for the determination of felodipine is 238nm where it gave a good sensitivity for the assay using UV-Visible spectrophotometer. The mobile phase was optimized to Buffer: Acetonitrile: Methanol in proportion 2:2:1 v/v respectively. The method was performed with flow rates 1ml/min. Temperature was maintained at Ambient. Standard deviation and %RSD were calculated and obtained as 0.3% respectively. LOD and LOQ were found to be 0.56μg/ml, 1.71μg/ml respectively. The Accuracy i.e, %Recovery for 50, 100 and 150% levels are obtained as 98.13%, 99.56%, 98.52% respectively. The % degradation results were within the limits and the method was validated.
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