Method development and validation of nifedipine and lignocaine by RP-HPLC

Abstract

For the validation of the nifedipine and lignocaine assay by reverse phase high performance liquid chromatography, in both pure form and tablet dosage form, a straightforward, quick, and exact approach has been established. ACN, Methanol, and perchloric acid were used as the mobile phase in chromatography on a Kromasil 100-5-C18 (4.6 x 150 mm, 5 m) column at a flow rate of 1.0 ml/min. 210 nm was used for detection. Nifedipine and lignocaine had retention times of 3.30 and 5.820.02 min, respectively. In the concentration range of 10–50 mg/ml of nifedipine and 20–100 mg/ml of lignocaine, the approach yields linear responses. The method precision for the assay result was less than 2.0%RSD, and the Nifedipine and lignocaine individual assays should be between 98% and 102.0%, respectively. The technique is helpful for pharmaceutical and bulk formulation quality control.