Method development and validation of ibuprofen obtained from the extraction of bougainvillea campanulata flowers by UV spectrophotometry, FTIR and TLC method

Abstract

The present study was aimed to develop and validate a simple, accurate, precise, reproducible UV-Visible spectrophotometric method and FTIR for the estimation of Ibuprofen present in extract. The solvent used in the experiment was m. Absorption maximum (λmax) of the drug was found to be 264 nm. The Beer’s law was obeyed in the range of 100-500 μg/ml. The method was shown linear in the mentioned concentrations having line equation y = 0.000023x with correlation coefficient r of 1 The amount of ibuprofen present in the extract was found to be 7 ug/ml. The recovery values for ibuprofen present in Bougainvillea Campanulata flowers extract ranged from 85.9%-87.6%. The percent relative standard deviation (RSD %) of interday precision was 51.35 % and intraday precision was 42.95 %. The limit of detection and limit of quantification was 0.9 μg/mL and 2.7 μg/mL. The percent relative standard deviation of robustness of the method was 37.43-45.86. Hence, proposed method was precise, accurate and cost effective. This method could be applicable for quantitative determination of the methanol present in Bougainvillea flowers extract.