Method development and validation for the estimation of famotidine in pure and tablet dosage form by derivative spectroscopy and uv-spectroscopy

Abstract

Two simple, precise, rapid, sensitive and accurate spectrophotometric methods have been developed for the estimation of famotidine in pure and in tablet dosage form. Method A: famotidine showed an absorbance maxima at 266 nm in methanol: water (80:20 V/V) for UV spectroscopy; the linearity was found to be in the concentration range of 4 - 20 μg/ml, correlation coefficient were 0.9993, the precision values were 0.32–1.38 with mean accuracy value 100.18–103.39 %, limit of detection 0.22 and limit of quantification 0.67. Method B: famotidine showed an absorbance minima at 254 nmin methanol: water (80:20 V/V) for first order derivative spectroscopy; the linearity was found to be in the concentration range of 4 - 20 μg/ml, correlation coefficient were 1.0, the precision values were 1.05–1.18 with mean accuracy value 97.33–102.05 % respectively, limit of detection 0.66 and limit of quantification 2.0. Both the methods were validated as per the International Conference on Harmonization (ICH) guidelines. The validated methods can be used for the quantitative analysis of famotidine in pure form and in tablet dosage form.