Abstract
A new, simple, accurate, and economical method was developed for simultaneous estimation of Lamivudine and Raltegravir by RP-HPLC. The chromatographic separation was achieved through Phenomenex C18 250mm × 4.6mm, 5µm as stationary phase and mobile phase was Methanol: Acetonitrile: Millipore Water (60:10:30) v/v ratio with the flow rate of 1ml/min at 292nm. The developed method was validated for the quantitative analysis of commercially available pure drugs. The instrument used was Shimadzu PDA-20AR, Lab solution software. The retention time for Lamivudine and Raltegravir were found to be 2.883 and 4.430 respectively. The linearity study of Lamivudine was performed by using concentration range of 1-20µg/ml and for Raltegravir concentration range was at 10-30µg/ml and correlation coefficient (r2) was found to be 0.999 and 0.9977 respectively. The developed method was validated in reference to ICH Guidelines. Specificity, Linearity, Accuracy, Robustness, Precision and Ruggedness obtained within the acceptance range.
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