Method development and validation for simultaneous estimation of aspirin, ramipril and atorvastatin by RP-HPLC method in its pure and tablet dosage form

Abstract

A simple, accurate, and precise method for estimating Aspirin, Atorvastatin, and Ramipril in Tablet dosage form was developed. Symmetry C18 (150 x 4.6 mm, 3.5) was used to run the chromatogram. At a flow rate of 1.0 ml/min, a mobile phase containing potassium dihydrogen ortho phosphate (pH adjusted to 3) and Methanol in the ratio 35:65 was pumped through the column. The temperature was kept at 30°C. The optimised wavelength chosen was 240.0 nm. Ramipril, Atorvastatin, and Aspirin had retention times of 1.803 minutes, 2.506 minutes, and 3.875 minutes, respectively. The percent RSD of Ramipril, Atorvastatin, and Aspirin were determined to be 0.99, 0.42, and 0.42, respectively. The method is linear for Ramipril concentrations ranging from 12.5 to 62.5 g/ml, Atorvastatin concentrations ranging from 160 to 800 g/ml, and Aspirin concentrations ranging from 5 to 25 g/ml. The method was accurate, with a percent recovery of 99.13 to 100 percent for Ramipril, 99.3 to 100.2 percent for Atorvastatin, and 99.3 to 100 percent for Ramipril, respectively. The developed method is robust after deliberate changes in flowrate and mobile phase composition with a percent RSD less than 2.