Abstract
Methionine restriction (MR) has been shown to reduce the age-induced inflammation. We examined the effect of MR (0.17% methionine, 10% kCal fat) and MR + high fat diet (HFD) (0.17% methionine, 45% kCal fat) on body mass, food intake, glucose tolerance, resting energy expenditure, hind limb muscle mass, denervation-induced atrophy and overload-induced hypertrophy in young and old mice. In old mice, MR and MR + HFD induced a decrease in body mass. Muscle mass per body mass was lower in old compared to young mice. MR restored some of the HFD-induced reduction in muscle oxidative capacity. The denervation-induced atrophy of the m. gastrocnemius was larger in animals on MR than on a control diet, irrespective of age. Old mice on MR had larger hypertrophy of m. plantaris. Irrespective of age, MR and MR + HFD had better glucose tolerance compared to the other groups. Young and old mice on MR + HFD had a higher resting VO2 per body mass than HFD group. Mice on MR and MR + HFD had a resting respiratory quotient closer to 0.70, irrespective of age, indicating an increased utilization of lipids. In conclusion, MR in combination with resistance training may improve skeletal muscle and metabolic health in old age even in the face of obesity.
Highlights
Methionine restriction (MR) has been shown to reduce the age-induced inflammation
high fat diet (HFD) induced an increase in body mass in young mice (p ≤ 0.036, Fig. 1a) and there was a trend towards significant increase in body mass in the old group (p = 0.056; Fig. 1b)
We investigated the impact of MR in young and old mice on a normal or high fat diet on body mass, food intake, glucose tolerance, resting energy expenditure, Succinate dehydrogenase (SDH) activity, hind limb muscle mass and denervationinduced atrophy and overload-induced hypertrophy
Summary
Methionine restriction (MR) has been shown to reduce the age-induced inflammation. We examined the effect of MR (0.17% methionine, 10% kCal fat) and MR + high fat diet (HFD) (0.17% methionine, 45% kCal fat) on body mass, food intake, glucose tolerance, resting energy expenditure, hind limb muscle mass, denervation-induced atrophy and overload-induced hypertrophy in young and old mice. Reduced energy expenditure (EE) as a result of lower physical activity and basal metabolic rate are associated with ageing and are risk factors for the development of s arcopenia[5,6]. In addition to reduced EE due to lowered physical activity levels, the age related loss of muscle mass decreases metabolic rate and further contributes to a decrease in physical activity. Both factors contribute to the gain in fat mass that in turn induces loss of muscle mass via release of inflammatory cytokines[7]. While all the interactions between obesity and sarcopenia are not fully understood, it is clear that dietary intervention and exercise are essential to counteract the loss of muscle mass of obese people with sarcopenia
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