Abstract
This cross-sectional study was designed to identify information on the frequency, antimicrobial resistance and species diversity of methicillin-resistant coagulase negative staphylococci (MRCoNS) among pet dogs and humans within households. Fifty five nasal swabs each from dogs and their owners were collected. MRCoNS were identified based on gram staining, culture on mannitol salt agar, biochemical tests, and mecA gene amplification. The antibiotic susceptibility of the isolates was assessed by a disc diffusion test. Uniplex and multiplex polymerase chain reaction (PCR) were employed for the species identification of MRCoNS and SCCmec typing, respectively. Species were further confirmed by MALDI-TOF-MS. The prevalence of MRCoNS was 29% in dog owners and 23.6% in dogs. Four different species of MRCoNS, Staphylococci saprophyticus (48.3%), S. haemolyticus (24.1%), S. warneri (17.2%), and S. epidermidis (10.3%), were detected. Two isolates each from dog owners and dogs showed a constitutive resistance to macrolide-lincosamide-streptogramin B (cMLSB) resistance, eight isolates each from dogs and their owners showed a macrolide-streptogramin B (MSB) resistance, and only two isolates from dog owners revealed an inducible resistance to macrolide-lincosamide-streptogramin B (iMLSB) resistance. SCCmec types were SCCmec type IV (55.2%), SCCmec type V (24.1%), SCCmec III (10.3%), SCCmec II (3.4%); two isolates were non-typable. MRCoNS are prevalent and genetically diverse in companion animals and humans. Different species of MRCoNS were found in dogs and their owners.
Highlights
This cross-sectional study was designed to identify information on the frequency, antimicrobial resistance and species diversity of methicillin-resistant coagulase negative staphylococci (MRCoNS) among pet dogs and humans within households
The antibiotic susceptibility of MRCoNS isolated from dog owners (n = 16) and dogs (n=13) revealed an almost similar resistance pattern to erythromycin (75% vs. 77%), cotrimoxazole (31.2% vs. 38.5%), and ciprofloxacin (18.8% vs. 23.1%)
This study provides information on the frequency, antimicrobial resistance and species diversity of MRCoNS within households, as well as on MRCoNS pet–human interaction
Summary
This cross-sectional study was designed to identify information on the frequency, antimicrobial resistance and species diversity of methicillin-resistant coagulase negative staphylococci (MRCoNS) among pet dogs and humans within households. Different species of MRCoNS were found in dogs and their owners. 1. Introduction with regard to jurisdictional claims in Staphylococcus epidermidis, a common coagulase negative Staphylococci (CoNS), has been implicated in human infections, and a majority of the strains circulating in hospitals has been estimated to be methicillin-resistant and resistant to several classes of antibiotics [1]. Staphylococci acquire methicillin resistance by the acquisition of staphylococcal chromosomal cassette mec (SCCmec), the mobile genetic element carrying mecA, which encodes altered penicillin binding protein (PBP2a) that mediates resistance to virtually all beta-lactam antibiotics [2]. Regardless of the pathogenic potential, MRCoNS have been identified as an important source of antibiotic resistance published maps and institutional affiliations
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