Abstract
The aim of this study was to present molecular and antimicrobial resistance characteristics of methicillin-resistant Staphylococcus aureus (MRSA) clonal complex (CC) 398 isolated from diseased dogs and cats in Thailand. A total of 20 MRSA isolates of 134 Staphylococcus aureus isolated from canine and feline clinical samples during 2017–2020 were CC398, consisting of sequence type (ST) 398 (18 isolates), ST5926 (1 isolate), and ST6563 (1 isolate) by multilocus sequence typing. spa t034 and staphylococcal cassette chromosome mec (SCCmec) V were predominantly associated with ST398. Intraclonal differentiation was present by additional spa (t1255, t4653), non-detectable spa, composite SCCmec with a hybrid of ccrA1B1+ccrC and class A mec complex, and DNA fingerprints by pulsed-field gel electrophoresis. The isolates essentially carried antimicrobial resistance genes, mediating multiple resistance to β-lactams (mecA, blaZ), tetracyclines [tet(M)], aminoglycosides [aac(6′)-Ie-aph(2′)-Ia], and trimethoprim (dfr). Livestock-associated MRSA ST398 resistance genes including lnu(B), lsa(E), spw, fexA, and tet(L) were heterogeneously found and lost in subpopulation, with the absence or presence of additional erm(A), erm(B), and ileS2 genes that corresponded to resistance phenotypes. As only a single CC398 was detected with the presence of intraclonal variation, CC398 seems to be the successful MRSA clone colonizing in small animals as a pet-associated MRSA in Thailand.
Highlights
Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant bacterium widespread in human and domestic animals, and is associated with a wide spectrum of infection, from superficial to invasive and systemic infections [1]
Human-related MRSA clones are a major population in small companion animals, and CC398 has been sporadically identified as a subpopulation [7,8,9]
This study presents MRSA CC398 isolated of canine and feline origin which were associated with diseases
Summary
Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant bacterium widespread in human and domestic animals, and is associated with a wide spectrum of infection, from superficial to invasive and systemic infections [1]. Nosocomial infections are caused by a particular subpopulation, known as hospitalassociated MRSA (HA-MRSA). Molecular epidemiological tools, such as multilocus sequence typing (MLST), reveal HA-MRSA clonal lineages in terms of clonal complex (CC) and sequence types (ST), including CC8 (ST239), CC22 (ST22), CC30 (ST26) [2]. Various MRSA clones have been identified in dogs, cats and horses. Human-related MRSA clones are a major population in small companion animals, and CC398 has been sporadically identified as a subpopulation [7,8,9]
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