Abstract
The rapid rise in resistance of Staphylococcus aureus to various antimicrobial agents is now a growing concern. The objectives of this study were to investigate the frequency of Methicillin Resistant S. aureus (MRSA) in different cases of infection, and to determine the pattern of antimicrobial susceptibility among the S. aureus originated from human in Mymensingh, Bangladesh. Covering a period of five months during July 2014 to November 2014, a total of 65 samples comprises of pus, wounds, eye lesions, burns were collected from the Community Based Medical College Hospital (CBMCH), Mymensingh, Bangladesh, and the samples were processed at the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202. Among the 65 samples, 20% (n=13/65) were confirmed as S. aureus on the basis of colony morphology, staining characteristics, biochemical properties, and Polymerase Chain Reaction (PCR) by amplification of nuc gene (amplicon size 279-bp). Among these 13 S. aureus isolates, 15.38% (n=2/13) were found to be resistant to Methicillin which was confirmed by antibiotic disk sensitivity test and PCR by amplifying MRSA specific mecA gene (amplicon size 533-bp). The antibiotic disk sensitivity test indicated that the MRSA isolates were completely (100%) resistant to Penicillin and Erythromycin. The MRSA represents a major challenge for treatment in hospital due to the emergence and spread of antibiotic resistant isolates among susceptible individual.
Highlights
Staphylococcus aureus, a Gram-positive coccus, is a member of the Firmicutes, and is frequently found in the human respiratory tract and on the skin [1]
Out of the 65 samples, S. aureus could be isolated from 20% (n=13/65) samples, and mecA was detected in 15.38% (n=2/13) isolates
The high level of multiple drug resistance shown by the Methicillin Resistant S. aureus (MRSA) isolates in this study is of great concern
Summary
Staphylococcus aureus, a Gram-positive coccus, is a member of the Firmicutes, and is frequently found in the human respiratory tract and on the skin [1]. Methicillin was one of most common types of antibiotics used to treat S. aureus infections. Methicillin belongs to a comprehensive class of antimicrobials, the β-lactam antimicrobials or βlactams. The mode of action is inhibition of bacterial cell wall biosynthesis. The 1st Methicillin Resistant S. aureus reported in UK just 2 years after the introduction of Methicillin in 1959. MRSA are often described as community associated or healthcare associated. The MRSA is only susceptible to Vancomycin but it has a carcinogenic effect
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
