Methanotrophic Biodegradation of Trichloroethylene in a Hollow Fiber Membrane Bioreactor

Abstract

Biodegradation of trichloroethlyene (TCE) in a hollow fiber membrane bioreactor was investigated using a mutant of the methanotrophic bacteria, Methylosinus trichosporium OB3b. Contaminated water flowed through the lumen (i.e., fiber interior), and the bacteria circulated through the shell side of the membrane module and an external growth reactor. In mass transfer studies with a radial cross-flow membrane module, 78.3-99.9% of the TCE was removed from the lumen at hydraulic residence times of 3-15 min in the lumen and the shell. In biodegradation experiments, 80-95% of the TCE was removed from the lumen at hydraulic residence times of 5-9 min in the lumen. The TCE transferred to the shell was rapidly biodegraded, with rate constants ranging from 0.16 to 0.9 L (mg of TSS) -1 day -1 . Radiochemical data showed that over 75% of the transferred TCE was biodegraded in the shell, with the byproducts being approximately equally divided between carbon dioxide and nonvolatiles. This study shows that a hollow fiber membrane bioreactor system coupled with the mutant strain PP358 of M. trichosporium OB3b is a very promising technology for chlorinated solvent biodegradation.