Methanotrophic bacteria occupy benthic microbial mats in shallow marine hydrocarbon seeps, Coal Oil Point, California

Abstract

Microbial mats composed of giant sulfur bacteria are observed throughout the benthos along continental margins. These communities serve to oxidize dissolved sulfides to sulfate, and are typically associated with the recent exposure of sulfide‐rich sediments. Such mats are also ubiquitous in areas of hydrocarbon seepage, where they are thought to consume sulfide generated in underlying sediment. Despite the high abundance of dissolved methane in hydrocarbon seeps, few studies have considered the importance of methanotrophy in mat communities. To assess the importance of methanotrophs in microbial mats from hydrocarbon seeps, an approach involving lipid biomarkers, stable isotopes and enrichment culturing was applied. Microbial mat samples were collected from benthic surfaces at two hydrocarbon seeps located in the Coal Oil Point seep field, offshore from Goleta, California. Both samples display a high abundance of 16:1 fatty acids, including two isomers specific to type I methanotrophic bacteria, 16:1(ω8) and 16:1(ω6). Depleted values of δ13C found in 16:1 fatty acids suggests methane assimilation into biomass, whereas three separate investigations of sulfide‐oxidizing bacteria yield fractionation factors too small to account for these values. On the basis of these observations and experiments, an isotope mass balance was applied to fatty acids present in the microbial mat samples which indicates methanotrophs contribute up to 46% of total fatty acids. These results implicate methanotrophy as an important function for microbial mats in seep areas, despite the visual appearance of these mats as being composed of giant sulfur bacteria.