Methanolic Phoenix dactylifera L. Extract Ameliorates Cisplatin-Induced Hepatic Injury in Male Rats.

Heba Nageh Gad El-Hak,Heba M A Abdelrazek,Heba M Elnegris,Hany Salah Mahmoud,Menna Allah I El-Menyawy,Mohamed T A Soliman,Tahany Saleh Aldayel,Eman A Ahmed

doi:10.3390/nu14051025

Abstract

This study investigated the ameliorative potential of methanolic date flesh extract (MDFE) against cisplatin-induced hepatic injury. Twenty male rats (weighing 180–200 g) were allocated into four groups: control; date flesh (DF) group (oral 600 mg/kg MDFE for 21 days); Cis group (7.5 mg/kg i.p. at day 16); and date flesh/cisplatin (DF/Cis) group (oral 600 mg/kg MDFE for 21 days and 7.5 mg/kg i.p. at day 16). Hepatic biochemical parameters in sera, and inflammatory and oxidant/antioxidant hepatic biomarkers were estimated. Hepatic histological changes and the immunohistochemistry of cyclooxygenase-2 (COX-2), nuclear factor kappa B (NF-κB), and alpha smooth muscle actin (α-SMA) were assessed. Pretreatment with MDFE decreased Cis-triggered liver biochemical parameters, oxidative stress, inflammatory biomarkers, and histological damage. Moreover, MDFE treatment reduced Cis-induced hepatic NF-κB, COX-2, and α-SMA protein expression. MDFE exerted a hepatoprotective effect when used concomitantly with Cis. Its effect was mediated via its antioxidant and anti-inflammatory ingredients.

Highlights

IntroductionChemotherapeutic agents have widely been used to slow and/or stop the development of cancer cells [1]
Metabolites found in both positive and negative modes showed that flavonoids, flavanones, anthocyanidin-3-O-glycosides, flavonoid-7-O-glycosides, cinnamic acids, hydroxycinnamic acids, and flavonoid-3-O-glycosides were present in the methanolic extract of date flesh (Table 1)
The human dose for methanolic date flesh extract (MDFE) would be equivalent to 97.2 mg/kg body weight according to Paget and Cisplatin-induced hepatic injury and inflammation manifested as elevations in enzymatic indices and histological lesions

Summary

Introduction

Chemotherapeutic agents have widely been used to slow and/or stop the development of cancer cells [1]. Cisplatin (Cis), cis-diammineplatinum (II) dichloride, is a platinumbased drug which is one of the most effective anticancer agents. It has been widely used to treat testicular, ovarian, cervical, bladder, neck, and lung cancers [2]. Cisplatin’s cytotoxic influences on cancer cells are thought to be attributable to its interaction with

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