Abstract
Herein, we report extraction of biologically important isolates from medicinally valuable plant Wedelia trilobata. The biological activities viz., anti-proliferative activity and cytotoxic effect of the methanolic extract of Wedelia trilobata was investigated by Thymidine uptake assay, wound healing assay and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, respectively. In addition, apoptotic activity was also examined through nuclear staining and DNA fragmentation assays. The IC50 value of the methanolic extract for the growth inhibition of MEG-01 cells was found to be 80 μg/ml under which the normal HEK-293 cells were resistant to the toxic effect. Concentration and time dependent studies in Thymidine uptake and wound healing assays revealed the prominent antiproliferative activity against MEG-01 at the concentration of 80 μg/ml for 48 h. The nuclear staining and DNA fragmentation assays also confirmed that the methanolic extract induces apoptosis in MEG-01 cells.
Highlights
Drugs for major human ailments including cancer are developed by natural products
The inhibition effect of methanolic extract on MEG-01 cell proliferation was rechecked by Thymidine uptake assay which confirmed the anti-proliferative effect of methanolic extract on MEG01 cells (Figure 2)
Inhibition effect of methanolic extract of Wedelia trilobata on wound reduction The adherent cell cultures of MEG-01 were utilized in the experiment that produced statistically significant result
Summary
Drugs for major human ailments including cancer are developed by natural products. Though many drugs of natural origin do exist, search for new anti-cancer agents is still necessary to increase the availability range and to find drugs less toxic while being more effective. Molecular extracts of medicinal plants have proven to be a reliable source of new and effective anticancer agents. These medicinal plants are rich in organic compounds and small molecules which are more stable with interesting biological activities and free from contaminants of chemical and biological origin. Majority of the world population is dependent upon traditional medicines. Considering the plants with medicinal values, Asteraceae is the largest family of flowering plants and renowned for its traditional medicine properties like Wedelia calendulacea plant possessing hepato-protective activity in which it revitalizes the liver and is used to treat liver dysfunction [1], methanol extracts of Wedelia trilobata are known to be having potent antioxidant and anti-bacterial activity [2], Vernonia cinerea used as an therapeutic agent in malarial fever and various gastro-intestinal disorders [3,4], Bidens alba possess anti-malarial activity in its ethanol extracts [5], Acourtia thurberi has shown reduction of the blood sugar level in mice [6], Jurinea consanguinea and Evax pygmaea are the other Asteraceae family plants having antioxidant properties [7,8]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
