Methanolic Extract of Musa Sapientum Surker Proffers Antioxidant Effects against Indomethacin-Induced Ulceration Oxidative stress in Wistar Rats

Abstract

Effect of methanolic extract of musa sapientum sucker (MEMSS) in indomethacin-induced ulceration in Wistar rats was investigated. Thirty albino rats (120-150 g) were divided into six groups. Negative and positive control rats (group 1 and 2) received no treatment and indomethacin (50 mg/kg) respectively. Group 3 rats received Omeprazole (0.8 mg/kg) 4 hours following indomethacin (50 mg/kg) intoxication. Groups 4, 5, and 6 received 5 mg/kg, 10 mg/kg and 20 mg/kg of MEMSS respectively 4 hour after indomethacin (50 mg/kg) induction. Treatment last for fourteen days and dosing was via oral route. Rats were anaesthetized and sacrificed 24 hours following last treatment. In our results, we scored ulcer induction by indomethacin using elevated biomarker enzymes. Indomethacin (50 mg/kg) administered alone increased (p< 0.05) hepatic and renal alanine and aspartate aminotransferases (LALT, LAST and KALT, KAST) by 311.2%, 170.8% and 123.7%, 109.7% respectively when compared with negative control group. Similarly, MEMSS significantly reduced the elevated LALT, LAST and KALT, KAST quantitatively when compared with positive control (IDM) group. MEMSS when administered at graded doses (5, 10 and 20 mg/kg) increased (p< 0.05) catalase levels, liver and kidney SOD levels (p< 0.05) when compared with control groups. 20 mg/kg of MEMSS increased liver and renal reduced glutathione by 69.6% and 32.7% respectively. In conclusion, MEMSS possesses antioxidant benefits against indomethacin-induced ulceration and oxidative stress in rats which could attracts further histomorphological studies.