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Abstract
One of the problems associated with the use of the bacterial gus A gene as a reporter is the presence of ‘endogenous GUS’ activity in some plant species. A recent paper [1] suggests that the addition of methanol to GUS reaction buffer specifically inhibits endogenous GUS activity while enhancing the activity of an introduced gus A gene. The results described in this paper suggest this phenomenon may be explained by the effect methanol has on cell membranes and demonstrate that it does not specifically inhibit endogenous GUS activity.
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