Methanol and ethyl acetate extracts of red fruit (Pandanus conoideus Lam.) short-red showed high of phenolic and radicals scavenging activities

Abstract

Many studies done in Red Fruit/RF as an antioxidant source, but there were some controversies because of the abundant fruit variety and growing place. Most researches focus on Red Fruit oil/RFO. In this work, we analyzed the antioxidant activity of Short-Red RF methanol/ME and ethyl acetic extracts/EE with multiple assays, then evaluated the relationship between phenolic content and antioxidant activity then phenolic effectiveness and antioxidant capacity. Total phenolic content/TPC and antioxidant activities (TAA, DPPH, ABTS, RP) were determined. The flavonoid identification was made using LCMS. RACI and PAC were calculated. EE showed significantly higher TPC (20.11μg GAE/ml extract) and scavenging activity for anion and cation radicals than ME (TPC= 9.88μg GAE/ml extract). The correlation between TAA and TPC (R2= 0.999) or TAA and radicals scavenging activity was significantly strong (R2= 0.997–1.000). EE had high and positive RACI (0.71). ME had higher PAC in TAA (1.82) and ABTS scavenging activity (4.75), whereas EE and ME had the same PAC in DPPH scavenging (2.08). The main phenolic identified in the extracts were taxifolin, quercetin, and quercetin-3-glucoside contributed in the radicals scavenging and also ferric ion reducing activity. Based on this study, Short-Red RF extracts are a potential anion and cation radical scavenger.