Methanol and Butanol Extracts of Paeonia lutea Leaves Repress Metastasis of Squamous Cell Carcinoma.

Yoshiki Mukudai,Seiji Kondo,Hideyuki Shigemori,Hiromi Motohashi,Kosuke Kato,Chihiro Ito,Miharu Fujii,Meilin Zhang,Satoru Shintani,Tatsuo Shirota,Sunao Shiogama,Kazunaga Yazawa

doi:10.1155/2016/6087213

Abstract

Squamous cell carcinoma (SCC) is one of the most common cancers of the head and neck region worldwide and is generally treated surgically in combination with radiotherapy and/or chemotherapy. However, anticancer agents have numerous serious side effects, and alternative, less toxic agents that are effective as chemotherapeutics for SCC are required. The Paeoniaceae family is widely used in traditional Chinese medicine. We examined methanol and butanol extracts of Paeonia lutea (P. lutea) leaves for their potential as an anticancer agent. Both extracts decreased the proliferation of SCC cells, induced apoptotic cell death, and modulated migration, adhesion, chemotaxis, and haptotaxis in an extracellular matrix- (ECM-) dependent manner due to altered expression of several integrin subunits. Subsequently, SCC cells were subcutaneously transplanted into athymic nude mice; the extracts reduced the metastasis of SCC cells but had little effect on the volume of the primary tumor or survival or body weight of the mice. The results suggest that the extracts may hold promise for preventing cancer metastasis.

Highlights

Squamous cell carcinoma (SCC) is one of the most common cancers of the head and neck region worldwide, with more than sixty thousand patients diagnosed each year [1]
Extracts of P. lutea Leaves Decreased Proliferation of SCC Cells in an Anchorage-Independent Manner, but No Effect Was Observed on Monolayer Cultures
We first investigated the effects of the P. lutea leaf extracts on the proliferation of SCC cells (Figure 1)

Summary

Introduction

Squamous cell carcinoma (SCC) is one of the most common cancers of the head and neck region worldwide, with more than sixty thousand patients diagnosed each year [1]. Treatment outcome is unsatisfactory, with 20% to 50% of patients developing regional recurrence and/or distant metastasis [2]. Anticancer agents often cause a variety of serious side effects; for example, cis-platinum (II) diammine dichloride (CDDP), which is often used for chemotherapy of SCC, causes nephrotoxicity [3], neurotoxicity [4], nausea, vomiting [5], ototoxicity [6], and xerostomia (dry mouth) [7, 8]. Several studies have reported anticancer effects of Paeoniaceae family preparations [18,19,20,21,22,23]

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Results

Conclusion