Abstract
Formaldehyde and NADH are formed from methanol and NAD in the reaction catalyzed by horse liver ADH and yeast ADH. In this reaction the above enzymes resemble closely human liver ADH, which has been known for some time to catalyze methanol dehydrogenation. The formation of formaldehyde from melhanol has been followed by chromatography of 2:4-DNPH derivatives of the reaction products and quantitation by the highly specific chromotropic acid method. Kinetic results have shown that turnover numbers × sec −1 × active site −1 are very similar for all three enzymes. The specificity of all three enzymes with respect to methanol is therefore identical. Human liver ADH, however, has the lowest K m for methanol, and on this basis only may be considered to be the best catalyst of this reaction.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
