Abstract
The effect of a limited supply of methane and oxygen on growth of Methylomonas flagellata was analyzed in chemostat culture. A significant decrease in YCH4 was observed under oxygen-limitation. Accumulation of formaldehyde is supposed to cause a decrease of YCH4. A low level of formaldehyde oxidizing activity in cells grown under oxygen-limited steady-states suggests that formaldehyde dehydrogenase is repressed under oxygen deficiency. In spite of the suppression of respiration, methane oxidation progressed independently under oxygen-limitation. These facts support the idea that methanol oxidation couples with methane oxidation (a mono-oxygenase system) in this microorganism.
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