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Abstract
Epithelial-mesenchymal transition (EMT), which is mainly recognized by upregulation of mesenchymal markers and movement of cells, is a critical stage occurred during embryo development and spreading cancerous cells. Metformin is an antidiabetic drug used in treatment of type 2 diabetes. EMT inhibitory effect of metformin has been studied in several cancers; however, it remains unknown in gastric cancer. The aim of the present study was to investigate the metformin effects on inhibition of EMT-related genes as well as migration and invasion of AGS gastric cancer cell line. Moreover, to study the effect of glucose on metformin-mediated EMT inhibition, all experiments were performed in two glucose levels, similar to non-fasting blood sugar (7.8 mM) and hyperglycemic (17.5 mM) conditions. The results showed reduction of mesenchymal markers, including vimentin and β-catenin, and induction of epithelial marker, E-cadherin, by metformin in both glucose concentrations. Furthermore, wound-healing and invasion assays showed a significant decrease in cell migration and invasion after metformin treatment in both glucose levels. In conclusion, our results indicated that metformin strongly inhibited EMT of gastric cancer cells in conditions mimicking normo and hyperglycemic blood sugar.
Highlights
Gastric cancer is the second leading cause of cancer-related mortality in the world [1]
Several molecular markers have been associated with the metastatic human cancers, one of the main agents contributing to tumor spread is a phenomenon, which is described as epithelial-mesenchymal transition (EMT) [2]
The aim of the present study was to investigate the effect of glucose levels on metformin-mediated inhibition of EMT in human gastric cell line
Summary
Gastric cancer is the second leading cause of cancer-related mortality in the world [1]. This high mortality rate arises from that a large number of patients are diagnosed at the advanced stages of gastric cancer when the tumor metastasis has been occurred. While detection of gastric cancer in early stages before its spread generally extends survival rate up to five-year higher. Several molecular markers have been associated with the metastatic human cancers, one of the main agents contributing to tumor spread is a phenomenon, which is described as epithelial-mesenchymal transition (EMT) [2]. EMT is naturally occurred during embryonic development, but recently, it has been become one of the especial research efforts.
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