Abstract
Electrospun nanofiber is an attractive biomaterial for skin tissue engineering because it mimics the natural fibrous extracellular matrix structure and creates a physical structure suitable for skin tissue regeneration. However, endowing the nanofibrous membranes with antibacterial and angiogenesis functions needs to be explored. In the current study, we aimed to fabricate gelatin/polycaprolactone (GT/PCL) (GT/PCL-Ag-Mg) nanofibers loaded with silver (Ag) and magnesium (Mg) ions for antibacterial activity and pro-angiogenesis function for wound repair. The fabricated GT/PCL membranes had a nanofibrous structure with random arrangement and achieved sustained release of Ag and Mg ions. In vitro results indicated that the GT/PCL-Ag-Mg membranes presented satisfactory cytocompatibility with cell survival and proliferation. In addition, the membranes with Ag demonstrated good antibacterial capacity to both gram-positive and gram-negative bacteria, and the Mg released from the membranes promoted the tube formation of vascular endothelial cells. Furthermore, in vivo results demonstrated that the GT/PCL-Ag-Mg membrane presented an accelerated wound healing process compared with GT/PCL membranes incorporated with either Ag or Mg ions and pure GT/PCL alone. Superior epidermis formation, vascularization, and collagen deposition were also observed in GT/PCL-Ag-Mg membrane compared with the other membranes. In conclusion, a multifunctional GT/PCL-Ag-Mg membrane was fabricated with anti-infection and pro-angiogenesis functions, serving as a potential metallic ion-based therapeutic platform for applications in wound repair.
Highlights
As the first barrier to bacterial invasion, skin tissue modulates body temperature and percepts noxious stimulation (Rahmani Del Bakhshayesh et al, 2018)
Electrospun technology has been widely applied in skin tissue engineering because of several fundamental features beneficial for rapid and functional wound healing and regeneration (Aavani et al, 2019)
Our results revealed that the GT/PCL nanofibers were successfully fabricated with random arrangement using wellestablished electrospinning conditions (Figure 1)
Summary
As the first barrier to bacterial invasion, skin tissue modulates body temperature and percepts noxious stimulation (Rahmani Del Bakhshayesh et al, 2018). Skin tissue has a selfhealing function after damage by trauma and burns, which is mainly mediated by three overlapping processes: inflammation, proliferation, and remolding (Xie et al, 2021). Some specific circumstances, such as large area skin defects or skin trauma in aging patients, cannot self-heal sufficiently to achieve structural and functional repair of the damaged skin tissue. Electrospun Nanofiber for Wound Repair (Qu et al, 2019). Delayed or incomplete skin repair will increase the risk of bacterial infection, increasing the psychological and physical burden of patients Developing wound care products that improve healing and prevent complications is needed in clinical practice
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