Abstract
Pyrophosphate (P2O74−, PPi) plays a vital role in ecological environment. Its elevated levels in water bodies can lead to eutrophication. Hence, its detection is extremely significant. Whereas most of the existing methods for the actual detection of PPi may cause environmental pollution or suffer from operational complexity. In this study, we introduced a sensitive and selective method for detecting PPi based on the fact that PPi can inhibit the peroxidase-like activity of adenosine 5′-triphosphate (ATP). This strategy not only eliminated the complexity of material preparation (ATP is commercialized), but also addressed the general need for metal ions in detecting PPi. The dynamic range of PPi detection was 1.0–200 μM and the detection limit was 74 nM. In addition, this strategy had been successfully applied to the determination of PPi in tap water and lake water. This work extends the application of natural biological small molecule ATP in the analysis and provides an innovative thought for the metal-free detection of PPi.
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