Abstract
Nowadays, bioactive nanomaterials have been attracted the researcher’s enthusiasm in various fields. Herein, Diplocyclos palmatus leaf extract-derived green-fluorescence carbon dots (DP-CDs) were prepared using the hydrothermal method. Due to the strong fluorescence stability, the prepared DP-CDs were coated on filter-paper to make a fluorometric sensor-strip for Fe3+ detection. After, a bandgap-narrowed DP-CDs/TiO2 nanocomposite (DCTN) was prepared using the methanolic extract of D. palmatus. The prepared DCTN exhibited improved photocatalytic bacterial deactivation under sunlight irradiation. The DCTN-photocatalysis slaughtered V. harveyi cells by the production of reactive oxygen species, which prompting oxidative stress, damaging the cell membrane and cellular constituents. These results suggest the plausible mode of bactericidal action of DCTN-photocatalysis under sunlight. Further, the DCTN has shown potent anti-biofilm activity against V. harveyi, and thereby, DCTN extended the survival of V. harveyi-infected shrimps during the in vivo trial with Litopenaeus vannamei. Notably, this is the first report for the disinfection of V. harveyi-mediated acute-hepatopancreatic necrosis disease (AHPND) using nanocomposite. The reduced internal-colonization of V. harveyi on the hepatopancreas as well as the rescue action of the pathognomonic effect in the experimental animals demonstrated the anti-infection potential of DCTN against V. harveyi-mediated AHPND in aquaculture.
Highlights
Nowadays, bioactive nanomaterials have been attracted the researcher’s enthusiasm in various fields
The relative intensity of the D-band and G-band was ID/IG = 0.842, which showed that the synthesized DP-CDs were partially amorphous because of the presence of hydroxyl functional groups
It is known that when plant extracts are hydrolyzed at high temperature, intermolecular and/or intramolecular dehydration will occur between the –OH, –COOH, and –H groups present in leaf extract and further polymerization, carbonization, and aromatization processes will lead to the formation of aromatic s p2 carbon[34]
Summary
Bioactive nanomaterials have been attracted the researcher’s enthusiasm in various fields. The DCTN has shown potent anti-biofilm activity against V. harveyi, and thereby, DCTN extended the survival of V. harveyi-infected shrimps during the in vivo trial with Litopenaeus vannamei This is the first report for the disinfection of V. harveyi-mediated acute-hepatopancreatic necrosis disease (AHPND) using nanocomposite. Iron (Fe3+) is the third most abundant element on the earth and considered as a crucial transition metal in cellular systems, which plays an important role in biological s ystems[3] Both deficiency and overabundance of F e3+ are associated with serious diseases in humans[4]. Some fluorescent sensors, such as organic dyes, quantum dots (QDs), metal nanoclusters, and metal–organic framework probes have been reported for metal sensing[6]. As the development of drug-resistance in bacterial pathogens due to the frequent use of antibiotics, a promising alternative approach is needed to control bacterial diseases in a quaculture[24]
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