Abstract
In the recent years the method of collecting and storing Dried Blood Spots (DBS) on special cellulose membrane (paper) has gained wide popularity. But possible damage of biosamples caused by microorganisms in case of their incomplete drying is a disadvantage of the method. It can be overcome by treating sample-collection membranes with colloidal solutions of metal nanoparticles, having antibacterial effect. The team studied antibacterial properties of nonwoven material samples with various coatings (alcohol sols of copper, aluminium, iron, titanium, silver and vanadium nanoparticles). Colloidal solutions of nanoparticles were obtained by means of electroerosion method with further low-temperature plasma condensation. Antibacterial activity of fiberglass and cellulose membrane samples with nanoparticle coatings was studied using B. cereus and plaque bacteria cultures. It was revealed that nanostructured coatings can suppress bacterial activity; in addition they can diffuse from the membrane surface into medium which leads to widening the areas of inhibiting testing cultures’ growth. Thus, membrane materials treatment with alcohol-sols of metal nanoparticles can be seen as promising for conferring antibacterial properties to DBS carriers.
Highlights
In the recent years the method of collecting and storing Dried Blood Spots (DBS) on special cellulose membrane has gained wide popularity
Tooth plaque bacteria and B. cereus bacilli were used as test cultures as the most resistant for disinfectology purposes
In the first experiment squares of the studied nonwoven materials as well as the control material square treated with silverbased nanodisperse system were placed in petri dishes on the surface of solid medium (SM) with test-culture inoculation (Figure 1)
Summary
In the recent years the method of collecting and storing Dried Blood Spots (DBS) on special cellulose membrane (paper) has gained wide popularity. It can be overcome by treating the collecting membranes with metal nanoparticles having antibacterial properties. Modification of membranes surfaces with nanomaterials and polymers with antibacterial properties is among most promising methods for maintaining stability of physico-chemical parameters of membranes.
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