Abstract

The goal in this study is to enhance the efficiency of bacterial extracellular electron transfer (EET) in Shewanella. oneidensis MR-1 by enhancing adhesion to the electrode. Our results clearly show a major difference in attachment and behavior of S. oneidensis MR-1 for Ca2+, Pb2+, Cd2+, and Mg2+ compared to the control. The final microbial coverage, as measured by confocal microscopy and cathodic peak charge in cyclic voltammetry (Qpc), increases with increasing metal ion concentrations. We found the cells attached to the electrode increased more with the addition of metal ion concentrations in the following order of metals: Ca2+ > Pb2+ > Cd2+ > Mg2+ compared to the control.Riboflavin production and glucose consumption mirrored the same order as the electrochemical results.

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