Metal Ion-Chelated Tannic Acid Coating for Hemostatic Dressing.

Bing Song,Lingyun Jia,Lulu Han,Liwei Yang

doi:10.3390/ma12111803

Bing Song, Lingyun Jia + Show 2 more

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https://doi.org/10.3390/ma12111803

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Abstract

Tannic acid (TA), a high-molecular-weight polyphenol, is used as a hemostasis spray and unguent for trauma wound remedy in traditional medical treatment. However, the use of tannic acid on a large-area wound would lead to absorption poisoning. In this work, a TA coating was assembled on a quartz/silicon slide, or medical gauze, via chelation interaction between TA and Fe3+ ions and for further use as a hemostasis dressing. Protein adsorption on the TA coating was further investigated by fluorescence signal, ellipsometry analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The adsorbed bovine serum albumin (BSA), immunoglobulin G (IgG) and fibrinogen (Fgn) on the TA coating was in the manner of monolayer saturation adsorption, and fibrinogen showed the largest adsorption. Furthermore, we found the slight hemolysis of the TA coating caused by the lysed red blood cells and adsorption of protein, especially the clotting-related fibrinogen, resulted in excellent hemostasis performance of the TA coating in the blood clotting of an animal wound. Thus, this economic, environmentally friendly, flexible TA coating has potential in medical applications as a means of preparing novel hemostasis materials.

Highlights

Polyphenols, which have traditionally been used for leather tanning and are globally referred to as “vegetable tannins”, are ubiquitous in plant tissues
Tannic acid (TA), a high-molecular-weight polyphenol containing five digalloyl ester groups covalently attached to a central glucose core, is found in a variety of places including as gall used in Chinese medicine, and in food products and stains
This may be due to the fact that the TA coating above 3 depositional cycles achieved full coverage on the surface of the substrate [16]. These results demonstrated that the TA coating, especially with multilayers, could act as a hemostatic dressing to control bleeding

Summary

Introduction

Polyphenols, which have traditionally been used for leather tanning and are globally referred to as “vegetable tannins”, are ubiquitous in plant tissues. Tannic acid (TA), a high-molecular-weight polyphenol containing five digalloyl ester groups covalently attached to a central glucose core, is found in a variety of places including as gall used in Chinese medicine, and in food products and stains. It exhibits antitumor, antibacterial, and antioxidant activity [3,4,5,6], but is used as a hemostasis spray and unguent for trauma wound remedies in traditional medical treatment [7,8].

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