Metal biosorption by magnetotactic bacteria isolated from fresh water sediments and characterization of extracted magnetosomes.

Abstract

The focus of the present study is to isolate and identify magnetotactic bacteria from fresh water sediments in Salem region, Tamil Nadu. Fresh water sediments were collected and characterized by X-ray diffraction to detect the crystalline nature of particles. Totally 12 isolates were screened for the metal biosorption efficiency in modified nutrient agar plates with 10mg (lower concentration) of four different metals viz., manganese chloride, zinc sulphate, copper sulphate and potassium dichromate. Followed by testing their ability to tolerate higher concentration of metals viz., 20mg, 30mg, 50mg, 70mg, 90mg and 150mg/50ml was analyzed. Only four bacteria survived the highest concentration of manganese and zinc (3000µg/ml), CuSO4 and K2Cr2O7 at a concentration of 1400µg/ml and 1800µg/ml, respectively. The four bacterial strains Stenotrophomonas maltophilia, two Pseudomonas aeruginosa strains and Achromobacter xylosoxidans were grown in modified nutrient broth (NB) and Luria Bertani (LB) incorporated with metals such as manganese, zinc, copper and chromium and tested for their efficacy to sustain metal stress. Since the two bacterial strains (SBY and KY1) were able to grow in both medium with a potential to with stand higher metal concentration these strains were further studied. A metal tolerant magnetotactic bacterial strain Pseudomonas aeruginosa SBY was confirmed by TEM analysis to detect the accumulated metal within the cell. As bacterial strains were capable of tolerating higher concentration of metal, they may have avital role in environmental bioremediation.