Metal and phytochelatin content in phytoplankton from freshwater lakes with different metal concentrations

Abstract

AbstractThe tace metal (Cu, Zn, Cd, Mn) and phytochelatin(an intracellular chelator for metal ions) cellular content were determined in phytoplankton samples originating from four lakes (Greifen, Sempach, Lucerne, and Orta). The lakes differ in their metal concentrations and in other conditions (pH, trophic state, organic matter). Total and intracellular contents of Cu and Cd were related to the experimentally determined free metal ion concentration and the total and intracellular content of Mn to the dissolved Mn. The intracellular Zn content was tightly regulated over a broad range of [Zn2+]. Phytochelatin concentrations were measurable in phytoplankton communities from three of the lakes, in spite of low levels of free Cu, Zn, and Cd ion concentrations. Culture experiments showed that the concentration of intracellular phytochelatin in Scenedesmus subspicatus and in a natural algal community increased upon addition of copper in a similar concentration range as in the lakes. Phytochelatin concentrations were below detection in the phytoplankton collected from the highly contaminated Lake Orta, perhaps suggesting that this algal community has adapted in some other way to high metal concentrations. Although we only sampled a few lakes, the lack of any clear relationship between phytochelatin and metal concentrations calls into question the feasibility of using phytochelatins as a bioindicator of metal exposure in lakes.