Metal and Pb isotope characterization of particulates encountered by foraging honeybees in Metro Vancouver

Abstract

Honeybees and their products are useful biomonitors of metal distribution in urban centres. This study investigates particulate sources that foraging honeybees encounter in Metro Vancouver. Metal concentrations and lead (Pb) isotope compositions were measured in topsoil (top 2 cm, n = 14) colocated with existing research hives and in particulate matter ≤10 μm (PM10, n = 27) collected throughout Metro Vancouver (British Columbia, Canada) during honeybee foraging hours over the course of one year (2018–2019). Topsoil served as a proxy for resuspended/coarse PM and, together with PM10, covered the size range of particulates collected by foraging bees both actively (pollen) and passively (dusts). Particulate matter ≤ 2.5 μm (PM2.5, n = 7) was collected on Whistler Mountain during two transpacific events (in spring 2014) to estimate the possible effect of transpacific particulate input on the Pb isotope composition of Western Canada aerosols. Metal concentrations and Pb isotopes in topsoil and PM from this study and bees and hive products from previous studies (collected in 2014–2019) reveal similar spatial trends: there were elevated amounts of some metals associated with anthropogenic activity (e.g., Pb, Zn, Sb) and less radiogenic Pb isotope compositions in most samples collected nearer to the city centre in comparison to samples collected in more suburban or rural areas. Bees and hive products have a smoothing effect on the spatiotemporal variability of the data; metal concentrations and Pb isotope compositions vary less in hive products than in PM, presumably because bees interact with multiple environmental domains while foraging. Wildfire smoke and transpacific input are phenomena that cause measurable shifts in Pb isotope compositions of PM, but not in hive matrices. The findings highlight important considerations to make (i.e., the smoothing effect) when linking public health data and decisions with environmental data from hive products in urban centres.