Abstract
Technologies such as next generation sequencing (NGS) are transforming research fields at the methodological, conceptual, and organizational level. They open up new possibilities and bring with them new commitments and inherent limitations. We show from a philosophy of science perspective how NGS-based metagenomics has transformed microbial ecology and, with it, parts of agricultural soil science, which integrate ecological approaches with the aim to inform agricultural practices. We reconstruct agricultural science as design science (sensu Niiniluoto) and describe how the possibilities, commitments, and limitations of metagenomics approaches in microbial ecology shape values, situation assessments, and recommendations for interventions of soil microbiology in the context of sustainable agriculture.
Highlights
RESEARCH ARTICLEMetagenomics approaches in microbial ecology and research for sustainable agriculture
A metagenomics approach consist in the sampling of genomic material directly from a selected environment
We show from a philosophy of science perspective how next generation sequencing (NGS)-based metagenomics has transformed microbial ecology and, with it, parts of agricultural soil science, which integrate ecological approaches with the aim to inform agricultural practices
Summary
Metagenomics approaches in microbial ecology and research for sustainable agriculture. Abstract Technologies such as generation sequencing (NGS) are transforming research fields at the methodological, conceptual, and organizational level They open up new possibilities and bring with them new commitments and inherent limitations. Wir rekonstruieren die Agrarwissenschaft als Designwissenschaft (sensu Niiniluoto) und beschreiben, wie die Möglichkeiten, Festlegungen und Beschränkungen der metagenomischen Ansätze in der mikrobiellen Ökologie die Werte, Situationsbewertungen und Empfehlungen für Eingriffe der Bodenmikrobiologie im Kontext nachhaltiger Landwirtschaft beeinflussen. Keywords basic vs. applied science, metagenomics, microbial ecology, sustainable agriculture
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