Abstract
Background: Throughout all of nature, microorganisms can be found thriving in a variety of environmental circumstances. Most marine life is either unculturable or challenging to culture using conventional techniques. Methods: Through the analysis of genomic data obtained directly from an environmental sample, metagenomics enables the study of all microorganisms, regardless of whether they can be cultured or not. This allows for the identification of the species present as well as the extraction of knowledge about the functionality of microbial communities in their natural habitat. Result: This article summarizes the state-of-the-art metagenomic approaches used and their associated softwares and tools. In order to comprehend and utilise unculturable microorganisms, metagenomics provides access to the enormous diversity of the microbial world and has significantly advanced both academic communities and industrial settings.
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