Metagenomic sequencing reveals detoxifying and tolerant functional genes in predominant bacteria assist Metaphire guillelmi adapt to soil vanadium exposure

Abstract

Due to extensive vanadium (V) mining and processing, an increasing amount of V has accumulated in soil, which poses a threat to public health. Consequently, we used earthworm (Metaphire guillelmi) incubation trials in V-contaminated soil (0–300 mg kg-1) to explore the response of soil indigenous bacteria and earthworm intestinal bacteria to V stress. Metagenomic analysis revealed that V exposure changed the bacterial composition in the soil and the worm gut. However, although the core species varied between soil and worm gut, the two systems shared the predominant bacteria, including Staphylococcus, Nocardioides, Streptococcus, and Nitrosopumilales. Two functional genotypes were detected in the shared core species, i.e., reductive genes and resistant genes. The reductive genes mainly consisted of those involved in glutathione, cysteine, methionine, sulfur, and nitrogen metabolisms. The resistant genes included those encoding the oxidation damage repair system, the outer membrane protein, the antioxidant enzyme system, the metal-binding, and the heavy-metal efflux. Therefore, the shared core species exert a comprehensive strategy to survive V stress involving the alliance of heavy metal detoxifying and tolerant genes. This study provides novel information about the detoxification mechanisms of bacterial populations in soil and worm gut to survive V stress.