Abstract
To explore the microbial nitrogen metabolism of a two-stage anoxic/oxic (A/O)-moving bed biofilm reactor (MBBR), biofilms of the system’s chambers were analyzed using metagenomic sequencing. Significant differences in microbial populations were found among the pre-anoxic, oxic and post-anoxic MBBRs (P < 0.01). Nitrospira and Nitrosomonas had positive correlations with ammonia nitrogen (NH4+-N) removal, and were also predominant in oxic MBBRs. These organisms were the hosts of functional genes for nitrification. The denitrifying genera were predominant in anoxic MBBRs, including Thiobacillus and Sulfurisoma in pre-anoxic MBBRs and Dechloromonas and Thauera in post-anoxic MBBRs. The four genera had positive correlations with total nitrate and nitrite nitrogen (NOX−-N) removal and were the hosts of functional genes for denitrification. Specific functional biofilms with different microbial nitrogen metabolisms were formed in each chamber of this system. This work provides a microbial theoretical support for the two-stage A/O-MBBR system.
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