Metagenomic insights into microbial diversity and carbon cycling related genes along the elevational gradient in an arid mountain ecosystem

Abstract

Understanding the elevational patterns of soil microbial carbon (C) metabolic potentials is instrumental for predicting changes in soil organic carbon (SOC) stocks in the face of climate change. However, such patterns remain uncertain in arid mountain ecosystems, where climosequences are quite different from other ecosystems. To address this gap, this study investigated the distribution determinants of microbial communities, C-cycling functional genes, and SOC fractions along the elevational gradient (1 707−3 548 m, a mean annual precipitation (MAP) range of 38 to 344 mm) of the north slope of Central Kunlun Mountain, using a metagenomic approach. Results showed that elevation significantly influenced the α-diversity (Shannon index) and composition of microbial communities as well as the C-cycling functional genes. The α-diversity of microbial taxonomy and C-cycling functional genes linearly increased with the increase in MAP along the elevational gradient. The elevational patterns of genes encoding glycoside hydrolases and glycosyltransferases (GTs) were mainly driven by electrical conductivity (EC), mean average temperature (MAT), MAP, and plant diversity. Furthermore, mineral-associated organic carbon (MAOC), particulate organic carbon (POC), and their sum generally increased with elevation. However, the MAOC/POC ratio followed a unimodal pattern, suggesting greater stability of the SOC pool in the mid-elevation regions. This unimodal pattern was likely influenced by the abundance of Actinobacteria and genes encoding GTs and carbohydrate esterases (CEs) and threshold effects of EC and MAT. In summary, our findings indicate that the distribution patterns of microbial communities and C-cycling functional genes along the elevational gradient in an arid ecosystem are distinct from those of regions with higher MAP, facilitating the prediction of climate change effects on SOC metabolism under more arid conditions. Soil salinity, plant diversity, precipitation, and temperature are the main regulatory factors of microbial C metabolism processes, and they potentially play a central role in mediating SOC pool stability.