Abstract
The ecological functions of forest soils are largely determined by the activities of soil microorganisms. This study aims to analyze the diversity and abundance of microbes in forest soil on Mount Ungaran, Ngesrepbalong Village, Kendal Regency, Central Java, Indonesia. This study used an exploratory observation method. Five hundred grams of forest soil was collected aseptically for chemical and metagenomic analysis. Metagenomic analysis was carried out based on 16S rRNA gene markers V3-V4 region. The result showing that the forest soil was dominated by bacteria of the order Rhizobiales (13.17%), Thermincolales (9.29%), and Vicinamibacterales (6.95%), as well as archaea of the order Nitrososphaerales (62.89%), Nitrosotaleales (4.61%), and Nitrosopumilales (1.67%). At the genera level, forest soil was dominated by Thermincola (8.42%) and Bacillus (7.39%), and one genera of archaea, namely Nitrososphaeraceae (23.17%). The bacteria that dominate forest soil are a group of Plant Growth Promoting Bacterial Agents (PGPB) in the nitrification process. Meanwhile, archaea that dominate forest soil act as ammonia oxidizing agents (AOA).
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