Abstract
Recent observations have been made regarding the generation of inositol 1,4,5-trisphosphate (IP 3), using chimeras of green fluorescent protein and the pleckstrin homology domain of phospholipase C- δ. In this paper a model is presented giving the quantitative relations between the green fluorescent protein–pleckstrin homology domain (GFP–PHD) construct and membrane phosphatidylinositol 4,5-bisphosphate (PIP 2) levels as well as the concentration of IP 3, the product of hydrolysis of PIP 2. The model can correctly reproduce the dependence of cytosolic GFP–PHD fluorescence on IP 3 concentration. This model extends a previous one (Metabotropic receptor activation, desensitization and sequestration—I: modelling calcium and inositol 1,4,5-trisphosphate dynamics following receptor activation, in this issue) dealing with the processes governing the production of IP 3 and the subsequent calcium (Ca 2+) changes in cells following activation of metabotropic receptors. This model is applied to the case of purinergic P 2Y 2 receptor activation in Madin–Darby Canine Kidney (MDCK) cells with adenosine triphosphate (ATP) (Science 284 (1999) 1527). It is shown that it can correctly reproduce the dependence of GFP–PHD fluorescence on the concentration of P 2Y 2 receptor ligand, as well as the temporal changes of GFP–PHD fluorescence following application of ligand.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
