Abstract
Abnormal reactive oxygen species (ROS) may mediate cytoplasmic male sterility (CMS). To observe the effect of ROS on soybean CMS, metabolite content and antioxidant enzyme activity in the flower buds between soybean N8855-derived CMS line and its maintainer were compared. Of the 612 metabolites identified, a total of 74 metabolites were significantly differentiated in flower buds between CMS line and its maintainer. The differential metabolites involved 32 differential flavonoids, 13 differential phenolamides, and 1 differential oxidized glutathione (GSSG) belonging to a non-enzymatic ROS scavenging system. We observed lower levels of flavonoids and antioxidant enzyme activities in flower buds of the CMS line than in its maintainer. Our results suggest that deficiencies of enzymatic and non-enzymatic ROS scavenging systems in soybean CMS line cannot eliminate ROS in anthers effectively, excessive accumulation of ROS triggered programmed cell death and ultimately resulted in pollen abortion of soybean CMS line.
Highlights
Cytoplasmic male sterility (CMS) is a maternally inherited trait that has been found in over 150 plant species [1]
Mitochondria are vital for producing ATP via the mitochondrial electron transport chain of the respiratory complexes [4], and its genome reorganization often leads to energy deficiency and abnormal accumulation of reactive oxygen species (ROS) in plant anther because mitochondria is the main organelle generating ROS [5,6]
Enzymatic ROS scavenging system usually consists of enzymes such as super oxide dimutase (SOD), catalase (CAT), peroxidase (POD) [14,18,19], and they are all abnormally expressed during pollen development in plant with CMS [6,14,15,16,17]
Summary
Cytoplasmic male sterility (CMS) is a maternally inherited trait that has been found in over 150 plant species [1]. Mitochondria are vital for producing ATP via the mitochondrial electron transport chain of the respiratory complexes [4], and its genome reorganization often leads to energy deficiency and abnormal accumulation of reactive oxygen species (ROS) in plant anther because mitochondria is the main organelle generating ROS [5,6]. Enzymatic ROS scavenging system usually consists of enzymes such as super oxide dimutase (SOD), catalase (CAT), peroxidase (POD) [14,18,19], and they are all abnormally expressed during pollen development in plant with CMS [6,14,15,16,17]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
